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Original Paper

Cluster N1 of complex I from Yarrowia lipolytica studied by pulsed EPR spectroscopy

T. Maly^{1, 3}, L. Grgic², K. Zwicker², V. Zickermann², U. Brandt² and T. Prisner¹

(1)

Institut für Physikalische und Theoretische Chemie and Center for Biological Magnetic Resonance, Johann-Wolfgang-Goethe-Universität Frankfurt, 60439 Frankfurt am Main, Germany

(2)

Zentrum der Biologischen Chemie, Universitätsklinikum Frankfurt, Molekulare Bioenergetik, 60590 Frankfurt am Main, Germany

(3)

Present address: Francis Bitter Magnet Laboratory and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

Received: 12 October 2005 Accepted: 16 January 2006 Published online: 26 February 2006

Abstract After reduction with nicotinamide adenine dinucleotide (NADH), NADH:ubiquinone oxidoreductase (complex I) of the strictly aerobic yeast Yarrowia lipolytica shows clear signals from five different paramagnetic iron–sulfur (FeS) clusters (N1–N5) which can be detected using electron paramagnetic resonance (EPR) spectroscopy. The ligand environment and the assignment of several FeS clusters to specific binding motifs found in several subunits of the complex are still under debate. In order to characterize the hyperfine interaction of the surrounding nuclei with FeS cluster N1, one- and two-dimensional electron spin echo envelope modulation experiments were performed at a temperature of 30 K. At this temperature only cluster N1 contributes to the overall signal in a pulsed EPR experiment. The hyperfine and quadrupole tensors of a nitrogen nucleus and the isotropic and dipolar hyperfine couplings of two sets of protons could be determined by numerical simulation of the one- and two-dimensional spectra. The values obtained are in perfect agreement with a ferredoxin-like binding structure by four cysteine amino acid residues and allow the assignment of the nitrogen couplings to a backbone nitrogen nucleus and the proton couplings to the β-protons of the bound cysteine residues.

Keywords Complex I - Iron–sulfur clusters - Ferredoxins - Electron spin echo envelope modulation - Hyperfine sublevel correlation

T. Prisner
Email: prisner@chemie.uni-frankfurt.de
Fax: +49-69-79829404

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